Departments

Mission

The mission of our department is to maximize the benefits of bioscience and biotechnology to our institute, environment and nation by being an excellent quality, comprehensive, multidisciplinary research, training and services center, that coordinates, disseminates and advances biotechnology and its applications for the benefit of society and human health. This will be through continuous learning to enhance the knowledge and skills that are of value to our staff.

Vision

Attain significant research heights and recognition as leader Molecular biology, genetic engineering, biotechnology and Nanobiotechnology research center. Shaping this research into innovative applicable tools for generation of knowledge, processes and products for benefit of the society and nation.

Research Fields

Gene isolation, cloning and characterization:
Including isolation of genetic materials, PCR, DNA sequencing, DNA labeling and modification, Northern blot and Southern blot, In situ hybridization, construction of genomic and cDNA expression libraries, ESTs development and gene Cloning.

Recombinant protein expression:
 in different E. coli systems, shake flask culture and fermentation, protein folding, one and 2 D protein electrophoresis, protein purification using chromatography and quantification, HPLC analysis, preparative isoelectric focusing and activity assays.

Immunological characterization:
Development of specific polyvalent antibodies, sera treatment, IgG purification, expression libraries immuno-screening, Western blot, dot blot, ELISA, immunohistochemistry and immuno-affinity chromatography.

–In-vitro and In-vivo culturing of hepatocytes:
-Hepatocytes culture, immuno-modulation and transplantation (in spleen and peritoneum).
-MDBK and VERO Cells, and vesicular stomatitis virus (VSV) for antiviral assays.

Bioinformatics:
Retrieve and analysis of DNA and protein sequences, data bases search, primer design, notification of unknown sequences, design of gene models for recombinant protein expression in different host cells.

Molecular diagnosis:
– PCR and real time PCR for detection and quantification of infectious agents (see routine services).
– In situ hybridization, reverse hybridization, array hybridization.

Designing of nano- and micro-carrier systems for Cargo delivery:
Synthesis and characterization of nano and micro capsules for controlled delivery of therapeutic drugs and genetic materials into targeted cells (synthesis of capsules, coating, functionalization, cargo loading, encapsulation efficiency, cargo release, cellular uptake, Investigation of the cytotoxic effects the capsules on cells, plasma stability, etc ).

Utilization of nanoparticles to develop novel diagnostic tests: 
For detection of biomarkers of diseases and pathogens ( Metallic NPs synthesis, characterization and Functionalization, NPs conjugation with oligonucleotide probes).

Techniques

Gene isolation, cloning and characterization:
Including isolation of genetic materials, PCR, DNA sequencing, DNA labeling and modification, Northern blot and Southern blot, In situ hybridization, construction of genomic and cDNA expression libraries, ESTs development and gene Cloning.

Recombinant protein expression:
 in different E. coli systems, shake flask culture and fermentation, protein folding, one and 2 D protein electrophoresis, protein purification using chromatography and quantification, HPLC analysis, preparative isoelectric focusing and activity assays.

Immunological characterization:
Development of specific polyvalent antibodies, sera treatment, IgG purification, expression libraries immuno-screening, Western blot, dot blot, ELISA, immunohistochemistry and immuno-affinity chromatography.

–In-vitro and In-vivo culturing of hepatocytes:
-Hepatocytes culture, immuno-modulation and transplantation (in spleen and peritoneum).
-MDBK and VERO Cells, and vesicular stomatitis virus (VSV) for antiviral assays.

Bioinformatics:
Retrieve and analysis of DNA and protein sequences, data bases search, primer design, notification of unknown sequences, design of gene models for recombinant protein expression in different host cells.

Molecular diagnosis:
– PCR and real time PCR for detection and quantification of infectious agents (see routine services).
– In situ hybridization, reverse hybridization, array hybridization.

Designing of nano- and micro-carrier systems for Cargo delivery:
Synthesis and characterization of nano and micro capsules for controlled delivery of therapeutic drugs and genetic materials into targeted cells (synthesis of capsules, coating, functionalization, cargo loading, encapsulation efficiency, cargo release, cellular uptake, Investigation of the cytotoxic effects the capsules on cells, plasma stability, etc ).

Utilization of nanoparticles to develop novel diagnostic tests: 
For detection of biomarkers of diseases and pathogens (Metallic NPs synthesis, characterization and Functionalization, NPs conjugation with oligonucleotide probes).

Services

Training

Biochemistry and Molecular biology Department at TBRI had kept steady record for holding local, national and international training workshops (hands on) in different aspects of molecular biology, biotechnology and Nanobiotecnology as follows:

1.  Biochemistry and Molecular Biology Department is considered by WHO as reference training center for African and Arabian countries concerning molecular diagnosis of infectious diseases
2.  Training courses in principles of molecular biology.
3.  Organizing workshops on molecular forensic diagnosis.
4.   Workshops on real time PCR.
5.   Workshops on Nanotechniques.
6.   Workshops in recombinant protein expression in E. Coli systems.
7.   In collaboration with universites, selected graduation research projects have been conducted at Biochemistry and Molecular biology Dept., TBRI.

Customized training workshops can be organized in many other different fields for groups.

Publications

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• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
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Projects

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with

• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,
• Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s,