Tissue Engineering and Cell-Based Regenerative Therapy Unit
About Specialized Unit
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The Tissue Engineering and Cell Regenerative Therapy Unit was established by the decision of the Board of Directors of the Theodor Bilharz Institute on January 28, 2009, under the supervision of Prof. Dr. Hanan El Baz (Professor of Immunology, TBRI).
The unit’s staff consists of professors, researchers, assistant researchers, and resident doctors.
The unit comprises five labs: the clinical research lab, the experimental research lab, the sample preparation and characterization lab, the molecular immunology lab, and the cryopreservation lab, as well as a storage area. Each lab is fully equipped with the latest modern appliances. Since 2009, several research projects have been carried out at the unit, including one funded by the STDF and 10 projects funded by the Theodor Bilharz Institute. Fifteen Master's and Doctoral theses have also been conducted. The results of this research have been published in international conferences and prestigious international journals.
Structure
structure
The unit consists of 6 labs:
- Clinical Research Lab:
A clean lab designed for clinical translation and applications of stem cell research. It consists of four areas:
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Area A (under the laminar flow): The most sterile area, used for isolation and culture of stem cells.
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Area B (Working Area): For processing samples. Benches are made of electro-polished stainless steel. This area is equipped with:
- Clini MACS (Miltenyi Biotech, Germany): A closed system used for clinical separation of different types of cells from bone marrow aspirate and leukapheresis.
- Sepax (Biosafe, Spain): A closed system used for separation of mononuclear cell layers from blood samples.
- CO2 incubator (Thermo Scientific, USA).
- Cooling centrifuge (Eppendorf, Germany).
- Laminar flow (Telstar, Spain).
- Balance.
- Shaker.
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Area C (Preparation Area): Contains benches and storage units made of electro-polished stainless steel. It is equipped with:
- Ordinary microscope (Olympus, Japan).
- Inverted microscope (Olympus, Japan).
- Digital camera with adaptor for ordinary and inverted microscopes (Olympus, Japan).
- Refrigerator.
- Laptop.
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Area D (Gowning Area): Contains a step-over bench, lockers, and a unit for storing sterilized gowns, all made of electro-polished stainless steel.
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Area E (Hand Sterilization Area).
- Experimental Research Lab:
Equipped with:
- Laminar flow (Telstar, Spain).
- CO2 incubator (Thermo Scientific, USA).
- Cooling centrifuge (Eppendorf, Germany).
- Ordinary microscope (Olympus, Japan).
- Inverted microscope (Olympus, Japan).
- Refrigerator.
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Sample Preparation Lab:
For paperwork and documentation. -
Molecular Immunology Lab:
For molecular characterization of stem cells and equipped with:
- Laminar flow (JSCB 1200SB, Korea).
- Real-time PCR (Applied Biosystem StepOne, USA).
- Thermal cycler (Bio-Rad, USA).
- High-speed cooling centrifuge (Eppendorf, Germany).
- Spectrophotometer (ABBOTT, USA).
- Gel electrophoresis.
- UV transilluminator.
- Microwave.
- Laptop.
- Cryopreservation Lab:
For storage of cells and tissues in liquid nitrogen (-200°C) and equipped with:
- Liquid nitrogen tank: A reservoir for liquid nitrogen.
- 2 Liquid nitrogen locators with racks (Thermo Scientific, USA): For storage of samples.
- Controlled-rate freezer (Thermo Scientific, USA).
- -80°C freezer (Thermo Scientific, USA).
- Store:
For storing sterile disposables.
Techniques
Isolation of stem cells
The research conducted at the unit focuses on isolating stem cells from various sources, followed by their propagation, differentiation, and transplantation. The goal is to study the therapeutic effects of these cells in various experimental models, including chronic liver diseases such as cirrhosis, malignant liver tumors, and colitis.
Services
The unit can assist researchers in the field of stem cells and tissue engineering through consultation or the conduct of research.
Contacts
Name : Prof. DR. Hanan el Baz Professor of Immunology, TBRI
- Helbaz77@gmail.com
- 01001724034