In addition to the highly experienced staff, Biochemistry and Molecular Biology Department at Theodor Bilharz Research Institute has a well equipped molecular biology and genetic engineering laboratory with well established domains and equipment for different specific fields such as, genetic material isolation, PCR, cloning, cell culturing, protein expression, protein purification, immuno-characterization (ELISA, Western, etc.) and bioinformatics. In 2005 we have established “Biotechnology and Genetic Engineering Unit” for the purpose of R & D in pilot scale production of important proteins especially biopharmaceuticals. This unit has fermentation facilities, protein purification and refolding facilities and tissue culture facilities for biological evaluation of activity of the produced recombinant proteins.NanoBiotechnology unit has been established in 2016 in to promote and support the development of new systems for diagnostics and disease treatment through the research in one of the most promising fields within the new technological advances: the nanoscience.
Major instruments are:
- Fermentor (BioFlo110, New Brunswick, USA). For bacterial cells and mammalian cells.
- French Pressure haemogenizer (Nero).
- Liquid chromatographic system (AKTA Purifier, GE Health Care).
- Ultra speed and cooling centrifuges.
- DNA extraction system and speed vacuum.
- Real time PCR and conventional PCR instruments (gradient, in situ).
- Nano Drop spectrophotometer instrument.
- CO2 incubators.
- Laminar flow cabinets.
- Agarose gel and PAGE electrophoresis systemsG
- Gel documentation systems
- One dimension and 2 dimension protein electrophoresis and transfer systems.
- Isoelectric focusing systems.
- FPLC chromatographic systems.
- Inverted phase and fluorescence and optical microscopes.
- Spectrophotometers.
- Deep freezers (-20 and -70) and autoclaving systems.
- Environmental shaker.
- ELISA reader.
